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× {{flash.message}} Toggle navigation Toggle navigation 日本語 | English 新規登録ログイン 齋田 良知 Yoshitomo Saita 更新日: 03/28 ホーム研究分野受賞論文MISC共同研究・競争的資金等の研究課題 論文 国際誌 2022年12月 Intra-Articular Injections of the Adipose-Derived Mesenchymal Stem Cells Suppress Progression of a Mouse Traumatic Knee Osteoarthritis Model. Cartilage Takanori Wakayama Yoshitomo Saita Masashi Nagao Sayuri Uchino Sei-Ichi Yoshihara Kunikazu Tsuji Hideyuki Koga Yohei Kobayashi Hirofumi Nishio Yasumasa Momoi Hiroshi Ikeda Kazuo Kaneko Muneaki Ishijima … 全て表示 折りたたむ 巻 13 号 4 開始ページ 148 終了ページ 156 記述言語 英語 掲載種別 研究論文（学術雑誌） DOI 10.1177/19476035221132262 OBJECTIVE: This study aimed to determine whether the intra-articular injection of human adipose-derived mesenchymal stem cells (ADSCs) protects against the progression of murine post-traumatic osteoarthritis. DESIGN: ADSCs were isolated from human abdomen or buttock adipose tissues. In in vitro study, ADSCs conditioned medium was added to human chondrocytes pre-treated with interleukin-1β (IL-1β), and resultant gene expression of target inflammatory genes was measured by real-time quantitative polymerase chain reaction. A mouse model of knee osteoarthritis was generated by unilaterally transecting the medial meniscus in the right hind limb of 20 female C57BL/6 mice. Mice were randomly assigned to 2 treatment groups that received 6 µl intra-articular injections of either phosphate-buffered saline (control) or 2 × 104 cells/μl of ADSCs 14, 28, and 42 days post-surgery. Mice were euthanized 84 days post-surgery and histological and micro-computed tomography evaluation of knee joints were analyzed. Hind limb weight-bearing distribution was measured pre-surgery and 28 and 84 days post-surgery. RESULTS: Conditioned medium from cultured human adipose-derived mesenchymal stem cells suppressed the expression of target inflammatory genes in chondrocytes pre-treated with IL-1β, suggesting anti-inflammatory properties (P < 0.01). Histological analyses indicated that the progression of destabilization of medial meniscus-induced knee osteoarthritis was suppressed by the administration of ADSCs compared with control group at medial femorotibial joint in vivo. This protective effect was related to a reduction in articular cartilage loss. CONCLUSION: The intra-articular injection of ADSCs suppressed articular cartilage loss in a mouse model of knee osteoarthritis, possible through anti-inflammatory mechanisms. リンク情報 DOIhttps://doi.org/10.1177/19476035221132262PubMedhttps://www.ncbi.nlm.nih.gov/pubmed/36314274PubMed Centralhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9924982 ID情報 DOI : 10.1177/19476035221132262PubMed ID : 36314274PubMed Central 記事ID : PMC9924982 エクスポート BibTeX RIS 論文リストへ メニュー マイポータル 研究ブログ資料公開 共著者の一覧 まだ共著者が1人も登録されていません。 {{coauthor.Related.name_str}} {{coauthor.Related.last_modified}} 更新 limit"> もっとみる 2017 researchmap 利用規約