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�{���փX�L�b�v �瓇�E���������J������@�i�����w �������͎����Z���^�[�j TEL. 0952-34-2431 ��849-8501 ���ꌧ����s�瓇5-1-1 ENGLISH Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University Research projects Development of genetically modified rabbits for human disease models Cryopreservation of rabbit sperm and embryo �@ Research members Teaching staff Shuji Kitajima, DVM, PhD, Associate professor Fumikazu Matsuhisa, DVM, PhD, Assistant professor Technical staff Mayo Kawabata Shigenobu Takuma Recent publications Katsuda, S., Horikoshi, Y., Shiomi, M., Kitajima, S., Ito, T., Hazama, A., Shimizu, T., and Shirai, K.: Arterial stiffness of the aorta and iliofemoral artery and their responses to nitroglycerin administration in myocardial infarction-prone Watanabe heritable hyperlipidemic rabbits. J. Hypertension 42(3): 441-449, March 2024. (DOI:10.1097/HJH.0000000000003608) Chen, Y., Yang, X., Kitajima, S., Quan, L., Wang, Y., Zhu, M., Liu, L., Lai, L., Yan, H., and Fan, J.: Macrophage elastase derived from adventitial macrophages modulates aortic remodeling. Front. Cell Dev. Biol., 10 January 2023, Sec. Stem Cell Research. Frontiers in Cell and Developmental Biology, Open Access, Volume 10, 10 January 2023, Article number 1097137. (DOI:10.3389/fcell.2022.1097137) Nishijima, K., Kitajima, S., Matsuhisa, F., Niimi, M., Wang, C-C., Fan, J.: Strategies for highly efficient rabbit sperm cryopreservation. Animals 11(5): 1220, 2021. Nishida, Y., Nishijima, K., Yamada, Y., Tanaka, H., Matsumoto, A., Fan, J., Uda, Y., Tomatsu, H., Yamamoto, H., Kami, K., Kitajima, S., and Tanaka, K.: Whole?body insulin resistance and energy expenditure indices, serum lipids, and skeletal muscle metabolome in a state of lipoprotein lipase overexpression. Metabolomics 17(3): 26, 2021. Matsuhisa, F., Kitajima, S., Nishijima, K., Akiyoshi, T., Morimoto, M., Fan, J.: Transgenic rabbit models: Now and the future. Appl. Sci. 10(21): 7416, 2020. Yan, H., Niimi, M., Kitajima, S., Zhou, H., Chen, Y., Wang, C., Matsuhisa, F., Yang, X., Yao, J., Yang, D., Zhang, J., Murakami, M., Nakajima, K., Wang, Y., Liu, E., Chen, Y. , Liang, J. and Fan J.: Apolipoprotein CIII deficiency protects against atherosclerosis in knock-out rabbits. Arterioscler. Thromb. Vasc. Biol. 40(9): 2095-2107, 2020. Chen, Y., Waqar, A.B., Nishijima, K., Ning, B., Kitajima, S., Matsuhisa, F., Chen, L., Liu, E., Zhang, J., Chen, Y.E., Liang, J., Fan, J.: Macrophage-derived MMP-9 enhances the vascular calcification and progression of atherosclerotic lesions in transgenic rabbits. J. Cell. Mol. Med. 24(7):4261-4274, 2020. Wang, C., Niimi, M., Kitajima, S., Matsuhisa, F., Yan, H., Dong, S., Liang, J., Fan, J.: Sex hormones affect endothelial lipase-mediated lipid metabolism and atherosclerosis. Lipids in Health and Disease 18:226, 2019. (Dec 23, 2019, Open access, article number 226) Niimi, M., Nishijima, K., Kitajima, S., Matsuhisa, F., Satoh, K., Yamazaki, H., Zhang, J., Chen, Y.E., Fan, J.: Macrophage-derived matrix metalloproteinase-1 enhances aortic aneurysm formation in transgenic rabbits. J. Biomed. Res., 33 (4): 271-279, 2019. Wang, C., Nishijima, K., Kitajima, S., Niimi, M., Yan, H., Chen, Y., Ning, B., Matsuhisa, F., Liu, E., Zhang, J., Chen, Y. E., Fan. J.: Increased hepatic expression of endothelial lipase inhibits cholesterol diet?induced hypercholesterolemia and atherosclerosis in transgenic rabbits. Arterioscler. Thromb. Vasc. Biol. 37(7): 1282-1289, 2017. Niimi, M., Yang, D., Kitajima, S., Ning, B., Wang, C., Li, S., Liu, E., Zhang, J., Chen, Y.E., and Fan, J.: ApoE knockout rabbits: a novel model for the study of human hyperlipidemia. Atherosclerosis 245: 187-193, 2016. Nishijima, K., Kitajima, S., Koshimoto, C., Morimoto, M., Watanabe, T., Fan, J., Matsuda, Y.: Motility and fertility of rabbit sperm cryopreserved using soybean lecithin as an alternative to egg yolk. Theriogenology 84: 1172-1175, 2015. Fan, J., Kitajima, S., Watanabe, T., Xuc, J., Zhang, J., Enqi Liu, E., Chen, Y.E. : Rabbit models for the study of human atherosclerosis: From pathophysiological mechanisms to translational medicine. Pharmacology ; Therapeutics 146: 104-119, 2015. Nishijima, K., Tanaka, M., Sakai, Y., Koshimoto, C., Morimoto, M., Watanabe, T., Fan, J., Kitajima, S.: Effects of type III antifreeze protein on sperm and embryo cryopreservation in rabbit. Cryobiology 69: 22-25, 2014. Nishijima, K., Yamaguchi, S., Tanaka, M., Sakai, Y., Koshimoto, C., Morimoto, M., Watanabe, T., Fan, J., Kitajima, S.: Effects of cholesterol-loaded cyclodextrins on the rate and the quality of motility in frozen and thawed rabbit sperm. Exp. Anim. 63(2): 149-154, 2014. 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