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× {{flash.message}} Toggle navigation Toggle navigation 日本語 | English 新規登録ログイン 細川 まゆ子 ホソカワ マユコ  (Mayuko Hosokawa) 更新日: 01/31 ホーム研究キーワード研究分野学歴論文MISC書籍等出版物講演・口頭発表等担当経験のある科目(授業)所属学協会Works(作品等)共同研究・競争的資金等の研究課題 MISC 2015年1月1日 The immunotoxic effects of fluoride on mice after subacute administration by evaluating cytokine mrna expression in splenocytes Fluoride Mayuko Hosokawa, Masashi Tsunoda, Yang Liu, Ken Nakano, Kazuyoshi Itai, Humio Tsunoda, Kazuhito Yokoyama, Yoshiharu Aizawa 巻 48 号 開始ページ 329 終了ページ 337 © 2015 The International Society for Fluoride Research Inc. The fluoride ion (F), an environmental pollutant, affects the cytokine mRNA expression of macrophages in vitro, but it is not clear whether or not these effects occur in vivo. In this study, we examined the effects of F on cytokine mRNA expression in splenic macrophages of mice after subacute oral administration for 1 mo as well as determining serum F. BALB/c mice were administered F at 0, 1, 5, 25, and 125 ppm in their drinking water. At the end of the 1-mo administration, the serum F was determined. Each spleen was sampled, the lymphocytes were separated as non-adherent cells, and the macrophages were separated as adherent cells. The macrophages were activated by lipopolysaccharide and the lymphocytes were activated by phytohemagglutinin. After the activation and incubation, the RNA was extracted and cDNA was synthesized. We examined the mRNA expressions of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNFα) for macrophages and those of interferon-γ (IFN-γ) and IL-2 for lymphocytes by RT-PCR (reverse transcription polymerase chain reaction). The mean intake of food or water per body weight was significantly lower in the 125-ppm group compared to the other groups. The relative weights of spleens in the 1- and 5-ppm groups were significantly lower than those in the control. The mRNA expression of TNFα in the macrophages was lower in the 125- ppm group. There were no differences among the groups for the mRNA expression of IL-1β in macrophages and those of IFN-γ and IL-2 in lymphocytes. Significant lower relative weights of the spleens were observed in a non-dose-dependent manner. Although there was no statistically significant difference in the expression of mRNA of TNFα in macrophages among the groups, the value in the 125-ppm group was lower. Although F may affect TNFα expression in vivo, the inability to demonstrate this in the present study may have been due to the F concentration in the blood not being sufficiently high compared with the value which affected mRNA expression in macrophages in vitro. リンク情報 URLhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84947742311&origin=inward ID情報 ISSN : 0015-4725SCOPUS ID : 84947742311 エクスポート BibTeX RIS MISCリストへ メニュー マイポータル 研究ブログ資料公開 共著者の一覧 まだ共著者が1人も登録されていません。 {{coauthor.Related.name_str}} {{coauthor.Related.last_modified}} 更新 limit"> もっとみる 2017 researchmap 利用規約